Journal: Biochemical and biophysical research communications

Article Title: Baicalein alleviates chronic acute stress-induced irritable bowel syndrome-like symptoms in rats via modulating the ODC1/NF-κB pathway and oxidative stress.

doi: 10.1016/j.bbrc.2025.152058

Figure Lengend Snippet: Fig. 6. Evaluation of baicalein’s impact on ODC1 expression in the colons of CAS-induced IBS rats via immunofluorescence analysis. Red: ODC1, Blue: DAPI. Scale bar = 200 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The membranes were then incubated overnight at 4 ◦C with specific primary antibodies: Ornithine decarboxylase-1 (ODC1) (1:200; Santa Cruz Biotechnology Inc., USA), nuclear factorkappa B (NF-κB) p65, p–NF–κB p65 (1:1000; Cell Signaling Technology, USA), β-actin, and GAPDH (1:1000; Beyotime, China).

Techniques: Expressing, Immunofluorescence

Journal: Biochemical and biophysical research communications

Article Title: Baicalein alleviates chronic acute stress-induced irritable bowel syndrome-like symptoms in rats via modulating the ODC1/NF-κB pathway and oxidative stress.

doi: 10.1016/j.bbrc.2025.152058

Figure Lengend Snippet: Fig. 7. Evaluation of baicalein’s impact on ODC1, NF-κB p65, and p–NF–κB p65 expression in CAS-induced IBS rats via Western blot assay. *P < 0.05 vs. C; **P < 0.05 vs. M; (n = 4).

Article Snippet: The membranes were then incubated overnight at 4 ◦C with specific primary antibodies: Ornithine decarboxylase-1 (ODC1) (1:200; Santa Cruz Biotechnology Inc., USA), nuclear factorkappa B (NF-κB) p65, p–NF–κB p65 (1:1000; Cell Signaling Technology, USA), β-actin, and GAPDH (1:1000; Beyotime, China).

Techniques: Expressing, Western Blot